Please use this identifier to cite or link to this item: https://hdl.handle.net/1/1363
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dc.contributor.authorForsyth, Cecily Jen
dc.contributor.otherNelson, M.en
dc.contributor.otherPopp, H.en
dc.contributor.otherHorky, K.en
dc.contributor.otherGibson, J.en
dc.date.accessioned2019-04-29T00:43:51Zen
dc.date.available2019-04-29T00:43:51Zen
dc.date.issued1994en
dc.identifier.citationVolume 10, Issue 2, pp. 55 - 59en
dc.identifier.issn0894-203Xen
dc.identifier.urihttps://elibrary.cclhd.health.nsw.gov.au/cclhdjspui/handle/1/1363en
dc.description.abstractA sensitive test for the presence of D-positive fetal red blood cells (RBCs) in the maternal circulation of D-negative women has been developed. It was used to investigate the possibility that the occasional failure in preventing alloimmunization might be due to the administration of inadequate amounts of prophylactic anti-D Rh immune globulin. The standard dose in Australia contains 125 microg of antibody, and can suppress immunization by an estimated 6 mL of packed D-positive RBCs. A fetomaternal hemorrhage (FMH) of this volume is detectable in the maternal circulation as approximately 0.25 percent of the total RBCs. Our test utilizes a commercially available human monoclonal IgG anti-D that has been biotinylated and used with a dye-conjugated streptavidin. Flow cytometry is used to quantitate fluorescing D-positive RBCs. To date, 2,288 tests have been performed on blood samples from D-negative women attending local antenatal clinics or at the time of delivery. Evidence for an FMH has been obtained in six cases (0.26%). In one case, the FMH was only 0.1 percent, and in another (confirmed by the Kleihauer-Betke method), fetal cells constituted only 0.2 percent. Additional Rh immune globulin was not given to these patients. In the other four cases, the D-positive fetal cells were estimated to be 0.7,0.5,0.5, and 0.4 percent, and additional prophylactic Rh immune globulin was administered. Although the prevalence of FMH is low, screening D- negative women at risk of alloimmunization has proved to be simple, fast, and inexpensive.en
dc.subjectHaematologyen
dc.subjectHematologyen
dc.subjectImmunologyen
dc.titleDevelopment of a flow cytometric test for the detection of D-positive fetal cells after fetomaternal hemorrhage and a survey of the prevalence in D-negative womenen
dc.typeJournal Articleen
dc.description.pubmedurihttps://www.ncbi.nlm.nih.gov/pubmed/15945791en
dc.identifier.journaltitleImmunohematologyen
dc.relation.orcidhttps://orcid.org/0000-0002-9108-3088en
dc.originaltypeTexten
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
crisitem.author.deptHaematology-
Appears in Collections:Haematology
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